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Brucellosis FAQ

February 2, 2026 By Arabelle Leave a Comment

What is it?
Brucellosis- AKA Brucella Canis or B. Canis is a highly contagious incurable disease caused by the bacterium Brucella Canis. Meaning, this is a bacterial infection that cannot be treated. It is the ONLY bloodborne pathogen recognized by OSHA in veterinary medicine.

What does it do?
Infection in male dogs can cause lead to testicular atrophy and infertility. Female dogs can abort in late gestation and have vaginal discharge. It can also result in litters that include dead, infected, and apparently healthy puppies. Dogs can also develop an infection within their spine, inflammation of the eyes, or non-specific signs such as lethargy, decreased appetite, and enlarged lymph nodes. Some dogs have no obvious clinical signs and cause infection for other dogs.

Why do we care?
Brucellosis is highly contagious, and resistant to antibiotics, making it untreatable. Brucella Canis can be present in saliva, urine, feces, and reproductive fluids and can live for several months in the right environment, as it can survive high humidity, low temperatures, without sunlight, dry surfaces, and can even survive the semen freezing process. With this, dust, dirt, water, clothing, and any other objects the infected dog may have been in contact with pose a risk of transmission. There is no cure for canine brucellosis, and dogs are considered infected for life, and there is also no vaccine available.

Brucella is zoonotic- which means you can get it too! Once in the human, the disease may never be eliminated – it hangs out in the bone marrow of the unfortunate recipient for the rest of their lives, causing symptoms that include fever, aches, and symptoms similar to that of influenza. It is of particular concern in the very young, the very old, patients who are immunocompromised. Women who are pregnant can lose a pregnancy – and when you consider most caretakers of dogs in shelters, humane societies, rescue organizations, dog breeding kennels are young females of child-bearing age, this is especially worrisome.

This is a reportable disease, and treatment is left up to your county officials- many will require that the entire kennel, and any dogs exposed, be euthanized.

The Society for Theriogenology recommends that all breeding dogs be tested based upon risk factors and frequency of breeding and found to be negative prior to breeding. This means both male and female dogs be tested prior to each breeding, and prior to semen freezing, all male dogs should be tested and found to be negative.

In addition, the Society for Theriogenology recommends that all dogs and dog semen imported into the United States are negative for B. canis.

There have been an increasing number of outbreaks throughout the United States, particularly in commercial breeding operations. As infected dogs move out of these facilities and co-mingle with other dogs as breeding stock and rescued dogs, brucellosis may easily spread into other breeding facilities and client’s homes. Dogs may be exposed by routes other than venereal transmission, such as through casual contact with urine and genital discharges at dog events and breeders who rescue dogs from breeding facilities.  Veterinarians and breeders must be less complacent about testing for this important and devastating disease.

For these reasons, we, and many other facilities, are now requiring brucellosis testing once a year for any studs being frozen with us.

Filed Under: Caution with your breeding dog, Stud Dog Management

Questions To Consider When Choosing a Potential Stud

February 2, 2026 By Arabelle Leave a Comment

Has the stud passed all health clearances (both physical & genetic) recommended for your breed? Health should always come first when planning a breeding.

Does the stud have a current brucellosis test? Always be sure both the stud and the bitch have been tested for brucella prior to breeding, regardless of whether it is a natural tie, side by side, or shipped semen.

Has the stud successfully sired a litter with the type of semen you are planning to use (fresh, chilled, or frozen) in the past?  If no, it is always a good idea to request an evaluation report from a collection done within the last 4 months when planning a fresh or chilled breeding. For fresh or chilled semen, the stud dogs semen count should be around 100 million sperm cells for every lolbs of body weight.

If you are looking at frozen semen, a report for the particular collection date you are purchasing should be requested. Be sure this report includes post thaw, abnormalities, and the number of live, motile, normal cells in each unit. When using frozen semen, the recommended dosage vary based on the breed, please feel free to reach out to us if you have any questions regarding recommendations for your particular breed.

Does this stud’s pedigree meet your needs? Is this stud’s temperament desirable and appropriate for for his purpose? Is there a family history of health, longevity, and performance titles? Always check the stud’s pedigree against your bitch’s to be sure it fits your needs and there is no overlap.


How many litters has this stud dog sired?
Choosing a proven stud is always a good idea, however, breeding a stud too frequently can reduce genetic diversity, and increase chances of inbreeding in the future. Overusing a particular stud can negatively affect the breed’s longevity.


Does your stud owner work with a reputable fertility clinic?
Proper collection and processing of the semen, whether it be side by side, fresh chilled, or frozen, is the first step towards a successful artificial insemination.


Does this stud meet breed standard?
No dog is perfect, however, you should try to choose a stud who compliments your bitch’s weak points, vice versa. Overdone studs with extreme features should be avoided.


Can you meet this stud in person?
While this is not always possible, it can be helpful to meet the stud you are considering in person, to be sure they are a good fit for your breeding program.


What is the stud fee?
Whether it is pick of the litter, or a fee, it is always good to be sure this is something you’re comfortable with.

Filed Under: Purchasing Semen, Stud Dog Management

Sirius Canine Fertility’s Tips for Importing Frozen Semen from Another Country

January 30, 2026 By Arabelle Leave a Comment

Thank you for considering us for your canine semen import. We are frequently asked “how much semen should I ask for” or “what is a breeding unit”?

These questions can be confusing, especially since there is no real standard between countries or even between veterinarians.

The following are the requirements we would like for our clients, to obtain the best chances for successful breeding.  These parameters are optimal, but it is certainly understandable if you are purchasing semen from a deceased stud dog, or if the semen is in short demand, to need to work with smaller numbers.

Semen is frozen by different methods, resulting in either “straws” or “vials”.  Vials are simple math, as they are usually divided into individual breeding units. One vial= one breeding unit.

Straws are generally 0.5 ml straws with a set amount of sperm in each, and the number required will depend on variables such as morphology and post thaw motility. Generally, we see a breeding unit of 3-5 straws.

Breeding units are where it gets extra tricky and there is no set standard.  The following is the recommended protocol for any imported semen.

  • A complete sperm count is done on the collection, and both morphology and post thaw motility being accessed when determining how to package the breeding unit.
  • We request a copy of the completed semenalysis prior to the importation to help with recommendations on how much semen to have sent over.
  • Regardless of the breed, we would like to see a minimum of 150 million live, normal, motile sperm cells, with the POST THAW MOTILITY included in the calculations. The preferred number is 200 million, but in smaller breeds, or when there is only a small amount of semen available, we can work with 150 million live, motile sperm per breeding unit.
  • We require the same number of live motile sperm per breeding unit, regardless of if the semen will be implanted via Transcervical insemination, or surgical implant.

When doing Transcervical insemination, optimum protocol is to do two inseminations, one breeding unit per insemination. With this in mind, we ask for a minimum of 2 breeding units, regardless of the method used to freeze the semen, whenever possible.  Due to the cost involved, it is worth having a discussion between the stud owner and purchasing bitch owner about sending an extra unit or two, in case the bitch does not take on the first breeding attempt.

  • If doing Surgical insemination, one breeding unit is used for the insemination. In many cases, a second breeding unit will be used for a follow up transcervical insemination.
  • If the semen is needing to be registered with the American Kennel Club, please note that we will need a small amount of semen to send in for DNA, if a swab is not included with the shipment, or if the stud dog is deceased. The DNA process requires 1 straw or 3 pellets to complete the DNA profile, so please send additional semen for the DNA testing.

Occasionally stud owners will send additional semen to us for storage in case of a missed breeding attempt.  We ask the stud and bitch owners to work out who’s account it will be stored under and who will continue with payments on the storage account going forward.

We hope this information is helpful when negotiating how much semen you will be purchasing. If everyone works with the same numbers and information, it increases chances for successful breeding and also keeps expectations uniform for all involved.

Please do not hesitate to ask us if you have any questions. We can be reached at export@siriuscaninefertility.com

Bridgett and Arabelle

Sirius Canine Fertility, Export Department

Filed Under: Purchasing Semen, Stud Dog Management

Optimizing Semen Quality in the Dog

April 28, 2015 By Bridgett Higginbotham 2 Comments

May 2015

Optimizing Semen Quality in the Dog

by Linda Montgomery DVM, PhD

Semen quality is a constant concern for dog owners.  Your dog may have achieved show or performance goals, at which time it is appropriate to collect and freeze his semen for future litters of champions.  Unfortunately many factors can adversely affect semen quality in the dog, including age, infections, diet, medications, environmental factors, and unrelated health conditions.

Most dogs experience a decline in sperm production with age.  We many times get a 10 or 12 year old dog whose owner wants to have collected and frozen.  Sometimes they still have sufficient numbers of sperm for this purpose, and sometimes they do not. What we recommend to dog owners is to collect and freeze their dogs when they are young.  Although this is an extra inconvenience and expense, it ensures that your dog’s legacy will be preserved.

[Read more…]

Filed Under: Stud Dog Management

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