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Home Blog Transcervical Insemination Success Rates: Our 2025...
Blog

Transcervical Insemination Success Rates: Our 2025 Data From 250+ Procedures

Arabelle Walik
Arabelle Walik
Sirius Canine Fertility
Transcervical Insemination Success Rates: Our 2025 Data From 250+ Procedures

Most reproductive clinics talk about experience. Very few publish their actual numbers. We think that’s backwards — if you’re trusting a facility with your breeding program, you deserve to see real outcomes from real cases, not vague claims.

Here’s our 2025 TCI data from over 250 transcervical inseminations performed at Sirius.


2025 Overall TCI Pregnancy Rates

Across all semen types and protocols, our overall pregnancy success rate was 88%. For breedings that followed our full progesterone timing protocol, that number climbed to 95%.

That gap between 88% and 95% tells you everything about why timing matters — and why we push so hard on it.


Frozen Semen TCI Results

Frozen semen is where most clinics see their numbers drop. It’s the hardest category to get right because post-thaw viability adds a layer of uncertainty that fresh semen doesn’t have. Our 2025 frozen semen results held strong.

Overall pregnancy success with frozen semen was 84%. When breedings followed our timing protocol, that rate jumped to 93%. Average litter size across both single and dual TCI protocols was 6 puppies per breeding.

For context, published literature typically places frozen semen conception rates between 50% and 70% for transcervical insemination. We’re consistently running well above that range.


Fresh Chilled Semen TCI Results

Fresh chilled semen — shipped from a stud dog at another location — performed at 84% overall and 94% with our timing protocol. Chilled semen loses motility during transit, so precise timing on the receiving end is critical. That 10-point spread between overall and protocol-timed breedings reinforces the same lesson: when timing is dialed in, success rates climb significantly.


Fresh Semen TCI Results

Fresh semen collected on-site gave us the highest numbers of the year, which is expected given that viability is at its peak. Overall success was 95%, and breedings on our timing protocol hit 98%.

At 98%, the margin for improvement is essentially zero. Fresh semen with proper timing is as close to a guaranteed outcome as reproductive medicine gets.


Why Progesterone Timing Is the Biggest Variable

The consistent pattern across every semen type is the same: breedings timed with our progesterone protocol outperform breedings that aren’t. The delta ranges from 3 points (fresh semen) to 9 points (frozen semen), and it’s largest exactly where it matters most — in the category where semen quality is most compromised.

Progesterone timing isn’t a suggestion. It’s the single highest-impact factor in whether a TCI breeding succeeds or fails.


How These Numbers Compare Year Over Year

Our 2025 rates are higher than the year before — which we didn’t think was possible. These aren’t small-sample flukes. Over 250 procedures across all semen types, performed by the same team, using the same protocols, with outcomes tracked case by case. The improvement reflects continued refinement in our timing protocols and technique, compounded over hundreds of breedings.


What This Means for Your Breeding Program

These numbers exist because of the breeders and veterinarians who trust us with their programs. Every data point represents a real dog, a real owner, and a real litter that made it to the ground. Nothing beats seeing those puppy photos come through — and nothing motivates a team like knowing the work is landing.

If you’re planning an upcoming breeding, call us at 530-273-9123. We’re here to help you maximize your chances with data-backed protocols and a team that takes your results personally.

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12444 Nevada City Hwy
Grass Valley, CA 95945

Office: 530.273.9123
Fax: 530.273.9128

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